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> 한국미생물생명공학회(구 한국산업미생물학회) > Journal of Microbiology and Biotechnology > 28권 12호

Urease Characteristics and Phylogenetic Status of Bacillus paralicheniformis

Urease Characteristics and Phylogenetic Status of Bacillus paralicheniformis

( Do-won Jeong ) , ( Byunghoon Lee ) , ( Hyundong Lee ) , ( Keuncheol Jeong ) , ( Mihyun Jang ) , ( Jong-hoon Lee )

- 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)

- 발행년도 : 2018

- 간행물 : Journal of Microbiology and Biotechnology, 28권 12호

- 페이지 : pp.1992-1998 ( 총 7 페이지 )


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초록(외국어)
In 2015, Bacillus paralicheniformis was separated from B. licheniformis on the basis of phylogenomic and phylogenetic studies, and urease activity was reported as a phenotypic property that differentiates between the two species. Subsequently, we have found that the urease activity of B. paralicheniformis is strain-specific, and does not reliably discriminate between species, as strains having the same urease gene cluster were identified in B. licheniformis and B. sonorensis, the closest relatives of B. paralicheniformis. We developed a multilocus sequence typing scheme using eight housekeeping genes, adk, ccpA, glpF, gmk, ilvD, pur, spo0A, and tpi to clearly identify B. paralicheniformis from closely related Bacillus species and to find a molecular marker for the rapid identification of B. paralicheniformis. The scheme differentiated 33 B. paralicheniformis strains from 90 strains formerly identified as B. licheniformis. Among the eight housekeeping genes, spo0A possesses appropriate polymorphic sites for the design of a B. paralichenofomis-specific PCR primer set. The primer set designed in this study perfectly separated B. paralicheniformis from B. licheniformis and B. sonorensis.

논문정보
  • - 주제 : 자연과학분야 > 생물
  • - 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
  • - 간행물 : Journal of Microbiology and Biotechnology, 28권 12호
  • - 발행년도 : 2018
  • - 페이지 : pp.1992-1998 ( 총 7 페이지 )
  • - UCI(KEPA) : I410-ECN-0102-2019-400-001361194
저널정보
  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1017-7825
  • - 수록범위 : 1991–2021
  • - 수록 논문수 : 5639