글로버메뉴 바로가기 본문 바로가기 하단메뉴 바로가기

논문검색은 역시 페이퍼서치

> 한국생약학회 > 생약학회지 > 49권 3호

LC-ESI-MS/MS를 이용한 평위산 주요 성분의 함량 분석

Quantitative Determination of the Marker Components in Pyungwi-San Using LC-ESI-MS/MS

서창섭 ( Chang-seob Seo ) , 신현규 ( Hyeun-kyoo Shin )

- 발행기관 : 한국생약학회

- 발행년도 : 2018

- 간행물 : 생약학회지, 49권 3호

- 페이지 : pp.270-277 ( 총 8 페이지 )


학술발표대회집, 워크숍 자료집 중 1,2 페이지 논문은 ‘요약’만 제공되는 경우가 있으니,

구매 전에 간행물명, 페이지 수 확인 부탁 드립니다.

4,500
논문제목
초록(외국어)
Pyungwi-san has been used to treat the digestive system diseases, physconia, nausea, anorexia, and dyspepsia in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was optimized for simultaneous determination of the 14 marker components, spinosin, liquiritirn apioside, liquiritin, narirutin, 6″′-feruloylspinosin, hesperidin, liquiritigenin, glycyrrhizin, 6-gingerol, atractylenolide III, honokiol, atractylenolide II, magnolol, and atractylenolide I in Pyungwi-san extract. All analytes were separated on a Waters Acquity UPLC BEH C18 analytical column (2.1×100 mm, 1.7 μm) with maintained at 45° C. The mobile phase consisted of 0.1% (v/v) aqueous formic acid and acetonitrile. The MS conditions were as follows: capillary voltage 3.3 kV, extractor voltage 3.0 V, RF lens voltage 0.3 V, source temperature 120℃, desolvation temperature 300℃, desolvation gas 600 L/h, cone gas 50 L/h and collision gas 0.14 mL/min. The coefficient of determination of 14 analytes was 0.9989-1.0000. The limits of detection and quantification values of the all analytes were 0.04-2.56 and 0.13-7.69 ng/mL, respectively. As a result of the analysis using the established LC-ESI-MS/MS method, the 5 components, spinosin, 6'''-feruloylspinosin, atractylenolide III, II, and I derived from Zizyphi Fructus and Atractylodis Rhizoma, were not detected in this extract. On the other hand, the 9 components except for the 5 components were 4.15-498.87 mg/kg in lyophilized Pyungwi-san extract. Among these components, glycyrrhizin, marker compound of Glycyrrhizae Radix et Rhizoma, was detected the most amount as a 498.87 mg/kg.

논문정보
  • - 주제 : 의약학분야 > 약화학
  • - 발행기관 : 한국생약학회
  • - 간행물 : 생약학회지, 49권 3호
  • - 발행년도 : 2018
  • - 페이지 : pp.270-277 ( 총 8 페이지 )
  • - UCI(KEPA) : I410-ECN-0102-2018-500-004128956
저널정보
  • - 주제 : 의약학분야 > 약화학
  • - 성격 : 학술지
  • - 간기 : 계간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : - / SCOPUS
  • - ISSN : 0253-3073
  • - 수록범위 : 1970–2021
  • - 수록 논문수 : 3326