Objective: To evaluate relationship between ultrasonographic fetal heart function and delivery mode. Methods: A prospective cohort study to investigate the association of fetal heart function with pregnancy and child outcomes is ongoing in a tertiary university hospital. This study was approved by institutional review board. For this case-control study, we reviewed the data on women who delivered singleton live-born infant between Feb 2020 and Jul 2020. We included all pregnant women who were undertaken fetal echocardiography in the second and third trimesters. Then cases that showed normal fetal health surveillance before delivery were selected. Demographic, obstetric and medical histories as well as prenatal echocardiographic records were collected. Results: A total of 32 women were enrolled during study period. Of these, 11 women had vaginal delivery and 21 had cesarean section. The indications of cesarean section were malpresentation of fetus, cephalopelvic disproportion, prior uterine surgery, failure to progress, and maternal request. Global longitudinal strain of left ventricle (LVGLS) was significantly lower in cesarean section group than in vaginall delivery group. (17.8 and 23.4, respectively, p = 0.008) Global longitudinal strain of right ventricle was marginally lower in cesarean section group than in vaginall delivery group. (17.2 and 22.9, respectively, p = 0.062) There were no significant differences in maternal age, body mass index, medical diseases, gestational age at delivery, birthweight, Apgar scores and abnormal neonatal outcomes between two groups. Even after adjusting for age and BMI (these are generally considered as confounding variable), LVGLS remained significant for the cesarean section group (odds ratio 1.269, 95% confidence interval 1.028-1.565, p = 0.026). Conclusion: LVGLS was significantly lower when cesarean section was performed compared with vaginal delivery. Our data suggest that prenatal fetal heart functions may be a parameter to predict delivery mode.