Objective: This study examined the hypothesis that endocrine disrupting chemicals (EDC) are associated with premature birth (PTB). Di-2-ethylhexyl phthalate (DEHP) and bisphenol A (BPA) are best known as endocrine disruptors. Although they are known to be linked to the pathogenesis of PTB, the molecular and cellular mechanisms have yet to be fully understood. Methods: Five-week-old, virgin female ICR mice were administered a bolus of 100ul sunflower seed oil (100%), DEHP, and BPA by intraperitoneal injection every three days for three weeks. Formalin-fixed paraffin uterine sections were stained with H&E. Protein and RNA were extracted from uterus using protein extraction solution and Tri-RNA reagent, respectively. Data analysis was conducted by using western blot and quantitative real-time polymerase chain reaction (qRT-PCR). Results: In the group that treated with DEHP and BPA respectively, the expression of cyclooxygenase-2 (COX-2), oxytocin receptor (OTR), and connexin 43 (CX-43) related to the onset of labor pain and delivery were obviously increased. These group also showed a significant increase in the mRNA levels of the pro-inflammatory cytokines tumor necrosis factor (TNF)-, interleukin (IL)-1, and IL-6. Conclusion: These results support that DEHP and BPA activate inflammatory signals and induce the expression of the uterine activation proteins. Together, this findings may provide insight into the association between EDC exposure and obstetrical complications.