페이지 로딩중.....
PaperSearch 학술논문검색사이트
 
  즐겨찾기에 추가 홈검색이용방법마이페이퍼고객센터    
검색 다국어입력
인기검색어:
 
 
로그인
회원가입(Sign up)ID/PWD 찾기
학회별 검색
간행물 검색
분야별 검색
상세검색
일자별 업데이트 현황
신규 등록 간행물
베스트논문
서비스학회 목록
 
 
 
Home>학술논문>검색결과
     
 


Gene expression profile of a persistently chronic wasting disease (CWD) prion-infected RK13 cell line

- 발행기관 한국예방수의학회(구 한국수의공중보건학회)
- 발행정보 Journal of Preventive Veterinary Medicine , 36 권 , 4 호 , Startpage 186 , Endpage 195 , Totalpage 10
- 저자 ( Min Jeong Kim ) , ( Hyun Joo Sohn ) , ( In Soo Cho ) , ( Won Yong Lee ) , ( Yoon Hee Lee ) , ( Dong Seob Tark ) , ( Hyo Jin Kim )
- 가격 3,500 원
- 발행년도 2012
- 주제키워드 Prion, RK13, elk, CWD, qRT-PCR
- 초록
Chronic wasting disease (CWD) is a neurodegenerative disorder in cervids and a member of the transmissible spongiform encephalopathies (TSEs), also known as prion diseases. We previously generated a persistently CWD prion infected RK13 cell line (RKC1-11) using elk PrPC expressing cells (elkRK13) that were generated with the lentiviral expression system. To investigate the differentially expressed (DE) genes involved in prion infection at the cellular level, we performed microarray analysis and identified the DE genes between CWD-infected (RKC1-11) and non-infected cells (elkRK13). Collectively, 88 genes were found to be differentially expressed (42 genes upregulated > 2-fold; 46 genes downregulated < 0.5-fold); additionally, 10 up- and 8 downregulated genes agreed with the results of the qRT-PCR. Among these genes, we chose 8 DE genes associated with cell growth, signal transduction, transport, immune response and apoptosis based on gene function analysis for further analysis. The expression of the selected genes was further validated using an animal model in normal- and CWD-infected TgElk mice showing clinical signs at 185 dpi. These identified DE genes in both the in vitro and in vivo model could help in understanding the diagnosis and pathogenesis of prion diseases.

미리보기 보관하기 구매하기 원문뷰어 무료설치
 
     
       
 
   개인정보보호정책 이용안내 사업자정보확인 광고 및 제휴문의뷰어 다운로드사이트맵