Maturity of pineapples has been evaluated subjectively based on sliced pineapple’s internal color which is time-consuming, costly and destructive. In this study, the feasibility of a Visible-Near infrared(Vis/NIR) transmittance spectroscopy was investigated for non-destructive analysis of pineapple’s maturity. For the experiment, Vis/NIR spectroscopy uses 4 light source that each light source power is 0.25 kW and detects internally reflected light. Thirty six pineapple samples were used for Vis/NIR spectroscopy with the spectral range of 400-1000 nm. For measurement of reference values of color, Each sample was sliced by 2 cm thickness, with a 2 cm gap from the center of the sample up and down. The color were measured using RGB camera and normalized using standard color chart. Spectral data from Vis/NIR spectroscopy were used for the development of pineapple’s maturity prediction models with a partial least square regression (PLSR) method. The PLSR models showed calibration R2 is 0.9, SEC(Standard error of calibration) is 9.1% ,cross-validation R2 is 0.83 and SECV(Standard error of cross-validation) is 12.1%. The results indicate that the proposed Vis/NIR spectroscopy has potential to be used for the maturity measurement of pineapple with nondestructive manner.