UV irradiation causes a photoaging, results in decrease of type I procollagen and increase of matrix metalloproteinase-1(MMP-1) in human skin and cultured dermal fibroblast. In this study, we investigated the effect of fish scale collagen peptide on type I prcollagen in cultured human dermal fibroblasts. After treatment of fish scale collagen peptide (Mw: c.a. 700 Da), the mRNA and the protein levels of type I procollagen was measured by real-time polymerase chain reaction and ELISA, respectively, in normal and UV exposure condition. We found that fish scale collagen peptide increased type I procollagen protein expression dose-dependently in human dermal fibroblasts. Our results suggest that fish scale collagen peptide plays an important role on recovering the type I procollagen expression in human dermal fibroblasts.