Background: Electrochemical lipolysis (ECL) is a new method to induce adipocyte damage using in situ hydrolysis of water into acid and base. Application of a direct current through passivated electrode after tumescence with saline can cause acid/base gradients by oxidation-reduction reactions, which damages adipocytes. Objectives: We investigated ECL use in ex vivo human adipose tissue. Methods: Human adipose tissue specimens were obtained following abdominoplasty. After injection of saline with tumescent technique, two platinum needle electrodes were inserted into each sample through a custom 3D-printedacrylic jig. Voltage was applied at a dosimetry of three to six volts exposed over five minutes. Specimens were hemisectioned through both electrode insertion sites and stained with pH sensitive dye. Computer software was used to calculate the spatial area of electrolytic acid base injury as a function of dosimetry. Samples were evaluated utilizing conventional histology and light microscopy. Results: Spatial pH distribution mapping revealed that localized electrolytic acid base injury varied appropriately with dosimetry. Histology showed localized injury with rupture of adipocyte plasma membrane, shrunken and retracted fat lobule, degeneration of adipocytes and hemorrhage with adjacent vascular damage. Conclusion: ECL caused injury in human adipose tissues. With future development, ECL may offer a low-cost, minimally-invasive method of destroying adipocytes for medical or cosmetic reasons.