Background: Fractional laser technology is routinely used in the treatment of acne scarring, with thermal injury resulting in collagen synthesis and remodeling. Objectives: Investigate whether use of a Fractional treatment with a wavelength 1,064-nm picosecond-domain laser is effective for the treatment of wrinkles and acne scars. Methods: Patients received 3 laser treatments. Laser treatment were performed with a 1064-nm picosecond laser with fractional hand-piece. At 8-week after treatment, the final assessment was performed. Results: Three individual dermatologists compared the blinded pre- and post-treatment photo to assess the clinical improvement. Skin topography and elasticity were measured using Antera 3dTM and CutometerTM respectively. Fifteen women and 7 men (mean age, 39 years; age range, 21-66 years) are enrolled including 12 wrinkle and 10 acne scar patients. There were statistically significant clinical improvements after treatment compared with baseline (3 weeks: 19.1%, p<0.01; 6 weeks: 25.9%, p<0.01; 14 weeks: 35.2%, p<0.01). Skin topography analysis including wrinkle, texture, depression and pores revealed significant improvement compared with the baseline However, skin elasticity showed no significant differences. Post-treatment erythema, mild edema, and petechiae were the only side effects noted. Conclusion: This study revealed the treatment of fractional 1064-nm picosecond laser is an effective way to achieve skin rejuvenation.