Inner nutshell of Castanea crerata(chestnut) has been used as an anti-aging and anti-wrinkle agent from the ancient time in east Asia. In order to develop new anti-aging and anti-wrinkle, ethanolic extract of inner nutshell of Castanea crenata(Cor-285) was prepared and various biological activities were evaluated. Cor-285 showed potent anti-oxidant activity. Especially, Cor-285 possessed potent free radical scavenging activity in vitro(lC₅₀:7.6㎍/ml) compared to gallic acid(IC₅₀:12.5㎍/ml). Cor-285 showed the preventive effect against UV-induced cytotoxicity of fibroblast at concentration of 25-250㎍/ml. When Cor-285 was evaluated for its anti-allergic activity, it effectively inhibited histamine release from mast cells induced by compound 48/80(86% inhibition at 10mg/ml). The inhibitory activity was stronger than that of glycyrrhizinate. Cor-285 also showed in vivo inhibition against delayed hypersensitivity as well as croton-oil induced ear edema in mice when topically applied. These results strongly suggest that Cor-285 may reduce immunoregulatory/inflammatory skin trouble. From the attempts to isolate the scavenger, were isolated. In a clinical trial of twenty healthy volunteers with aged skin, 6 weeks application of Cor-285(3% cream) decreased wrinkle about 26% and increased moisturizing 20% on the skin. All of these results indicate that Cor-285 may be an effective anti-aging and anti-wrinkle agent.