Background: With the increase in the prevalence of diabetic foot ulcer (DFU), the demand for optimal treatment and management strategies has risen too. Objectives: Our study investigated the wound-healing effect of platelet-rich plasma (PRP) on DFU and the correlations between the various factors affecting DFU treatment. Methods: 10 diabetes patients with grade 1 DFU were treated using PRP prepared from the patients’ blood (collected using a commercial kit, MyCells Autologous Preparation kit^®, Holon, Israel). The ulcer margins were biopsied before and after PRP treatment. Hematoxylin and Eosin staining and immunohistochemical staining for HIF-1α and CD31 were performed to confirm angiogenesis. Spearman’s correlation tests were performed to determine the correlations between the treatment period and various clinical factors. Results: The mean PRP treatment period was 29.4 days. PRP was administered a mean of 3 (1-6) times. The time taken for a 50% reduction in the wound area was 16 days. Among the clinical factors, the presence of calluses in the lesion prolonged the pretreatment period, and significantly reduced the duration of PRP treatment. After PRP treatment, on CD31 staining, the number of endothelial cells was significantly increased. However, on HIF-1α, there was no significant difference. Conclusion: We found that PRP can contribute to wound regeneration through neovascularization. PRP therapy, with various growth factors, is expected to be another treatment option for DFUs.