This study investigated changes in neck skin appearance middle-aged women in their 40s and 50s after ingesting probiotic or paraprobiotic Lactobacillus rhamnosus KCTC 5033. Women in each group consumed one capsule (1 × 10¹⁰CFU) for 3 months and underwent measurements of changes in neck skin appearance. Melanin content was significantly increased only in the placebo control group, whereas the moisture content was not altered in either the probiotic or paraprobiotic group. in the probiotic group. The erythema index was not changed, but oil content and roughness tended to increased oil content and decreased roughness in the probiotic and paraprobiotic groups compared with the findings prior to the experiment. Moisture was increased in both the probiotic and paraprobiotic groups, whereas moisture evaporation was significantly increased only in the control group. The neck skin surface temperature was only changed in the probiotic group. Therefore, the probiotic and paraprobiotic treatments inhibited melanin production by altering the intestinal condition, in addition to increasing moisture by increasing the oil content and decreasing skin roughness. In fact, subjects in these groups expressed greater satisfaction after the experiment, especially those in the paraprobiotic group. Thus, L. rhamnosushas been identified asa potential therapeutic for improving the appearance of skin for the first time.