Probiotics has drawn a great attention as an inner-beauty material, and its diverse effects through intestinal immunity balance have been reported. The study aims to examine the effects of Lactobacillus reuteri KCTC 3594 intake on skin health of 30-40 middle-aged women in a 12-week treatment. Thirty female volunteers were randomly divided into the three groups of 10 people; control group(hereinafter referred to as Cont), L. reuteri probiotics group(Pro) and L. reuteri paraprobiotics group(Para). L. reuteri was administered to each group (1×10¹⁰ CFU/capsule) on an empty stomach in the morning once a day for 12 weeks. After 12-week intake of L. reuteri, the amount of melanin was increased in all groups with statistical significance(p<.001) while no change was found in all groups in terms of facial erythema. Regarding TEWL, no significant change was observed in a Cont group while a statistically significant decrease was detected in Pro and Para groups(p<.001, p<.05). In terms of skin depression, no significant change occurred in a Cont group while a statistically significant decline was found in both Pro and Para groups(p<.01, p<.05). In overall, Pro and Para groups were effective in improving TEWL and skin depression. In terms of significant difference, a Pro group was greater than a Para group. This study confirmed that L. reuteri probiotics and paraprobiotics would be useful as an inner-beauty material which can strengthen skin health.