This study was conducted to analyse antibiotic activity of essential oils to Candida albicans which is causative agent in fungal vaginitis. Antibiotic activity of 56 essential oils collected in Korea was tested against Candida albicans KCTC 7965 by disc diffusion method, broth dilution method and MTT metabolic assay. Among the 56 well known essential oils that can be easily collected, only 41 showed a positive result on antibiotic activity, whereas the other 15 types did not show any results at all. A further classification of antibiotic activity for the 41 essential oils by diameter of inhibitory zone, showed that: 12 types of the 56 fall into a range of IZ ≤ 1 cm (+); 11 types a category of 1 cm< IZ ≤ 1.5 cm(++); 9 types a category of 1.5 cm< IZ ≤ 2 cm(+++), 4 types a category of 2 cm< IZ ≤ 2.5 cm (++++); and finally, 5 types a category of IZ >2.5 cm(+++++), respectively. In order to measure the antibiotic strength on those 5 most effective essential oils(+++++) against Candida albicans, we adopted the broth microdilution method as well as MTT metabolic activity assay, and compared with the commercially available antibiotic, Amphotericin B. After comparing and matching the MIC values of each essential oil and Amphotericin B, the result showed that the essential oils ranged from 0.0225-0.12% with the MIC50, whereas Amphotericin B with the same MIC value fell into a category of 0.3-0.7%. With the value of MIC0, essential oils showed a ranging 0.06-0.2%, compared to Amphotericin B`s 3-6%. As a result of those conducts, it is not hard to say that those 5 types of natural essential oils outperformed than those commercially available Amphotericin B, alluding the possible use as an antibiotics for Candidiasis in the future.