In this experiment, applicability of biogrouting method was evaluated at two construction sites. Comparison is made among the typical grouting, a patented grouting, and the biogrouting to evaluate the injection efficiency such as disparity effect and regenerating the functions of irrigations. Microorganism liquid and reaction liquid were separatly injected when implementaion of biogrout is difficult in actual sites. For ease of using biogrout, powdered products similar to cement were developed to use as injection material.