Background: Cryolipolysis is a noninvasive method for the selective reduction of localized fat tissues. It has demonstrated efficacy in both clinical and preclinical trials; however, despite its popularity, its mechanisms of action and evaluation methods are not yet fully defined. Objectives: The purpose of this study was to improved methods for cryolipolysis using a porcine model. Methods: The abdomens of female Prestige World Genetics (PWG) micro-pigs were treated with a cooling device (CRYOLIPO II™), and we examined photography, three-dimensional photography, ultrasound, gross, and microscopic pathology, and serum lipid level analyses in order to determine the mechanism of action, efficacy, and safety of the device. Results: CRYOLIPO II™ reduced abdominal fat in porcine model. Gross and microscopic histological results confirmed the noninvasive cold-induced selective subcutaneous fat destruction, and showed increases in pre-adipocyte differentiation and in the activation of lipid catabolism. In particular, we found that the device may increase PPARδ (delta) levels in adipose tissue at 30.60 days post-treatment. Conclusion: Fat reduction by cryolipolysis was achieved in our porcine model. Thus, our findings indicate that CRYOLIPO II™ may be a promising fat reduction device for body contouring and fat reduction in humans, and that cryolipolysis exerts its effects, at least partly, by targeting the PPARδ signaling pathway.