Background: Adiponectin has been known to reduce premature senescence through functional recovery of energy metabolism. However, these precise mechanisms of adjustment of aging process and enhance of skin barrier such as antimicrobial barrier are unclear on keratinocyte. Objectives: We elucidate the effect of Adiponectin in UV-induced senescence and dysregulated production of hBD2 in normal human keratinocyte. Methods: A human normal keratinocyte senescence in vitro model system was constructed by repeated low dose UVB exposure. The aging condition was determined by senescence-associated beta-galactosidase (SA-モ-gal) staining. The expression of mRNA level and protein level of materials related to premature senescence was analyzed by RT-PCR and Western blot each. Results: In photoaging condition, increase of SA-モ-gal activity and overexpression of hBD2 were shown. We also found that adiponectin induces decrease of SA-モ-gal activity and down-regulation of hBD2 mRNA expression through suppression of p65 and AP-1 transcription factor (c-jun) via p38 and JNK/SAPK in human keratinocyte.Conclusion: Adiponectin suppresses dysregulated production of hBD2 by attenuation of p38 and JNK/ SAPK MAPK signaling in aging process. Therefore, it could be developed as a therapeutic application for anti-photoaging treatment.