In order to develop an antimicrobial skin care cleanser using omija (Schisandra chinensis) extract, antibacterial tests against common bacteria E. coli, S. epidermidis and P. acnes related to acne, C. albicans causing dermatitis and vaginitis, and P. ovale causing dandruff were performed. Although omija flesh has been used for food, there is no previous study on antibacterial activities of omija flesh and seed separately. Thus, flesh and seed from omija were extracted with 60% and 95% ethanol respectively and used for antibacterial tests. The antimicrobial activity of omija was stronger in flesh extract compared to seed extract, and 95% ethanol extract had similar or somewhat higher activity compared to 60% ethanol extract. In addition, the flesh extract with 95% ethanol did not show antibacterial activity against S. epidermidis. The omija extract had the highest antimicrobial activities against P. acnes, followed by P. ovale, E. coli, S. epidermidis, and C. albicans. Only ethanol extract showed antimicrobial activity but water extract did not. Therefore, if water soluble materials of omija are used for food firstly and the used omija leftover can be extracted with ethanol, an antibacterial cleanser may be produced economically.