Twelve triterpenoids (1 - 12) were isolated from CHCl3-soluble fraction of fruiting bodies of Ganoderma lucidum. Extensive spectroscopic and chemical studies established the structures of these compounds as butyl lucidenate P (1), butyl lucidenate E2 (2), butyl lucidenate D2 (3), butyl lucidenate Q (4), ganoderiol F (5), methyl ganoderate H (6), methyl ganoderate J (7), lucidumol B (8), ganodermanondiol (9), methyl lucidenate N (10), methyl lucidenate A (11) and butyl lucidenate N (12). All of the compounds were examined for their cytotoxic activity against HL-60, HeLa, and MCF-7 cancer cell lines. Among them, compounds 4 and 8 showed cytotoxic activity with IC50 values of 6.6 and 1.6 μM against HL-60, respectively. In addition, compound 8 also showed cytotoxic activity with IC50 values of 2.0 μM against HeLa cancer cell line, other compounds were moderate or inactive.