We compared the pregnancy outcomes and uterine artery pulsatile index(PI) between normal group and hyperglycemic group at 50 g glucose challenge test (GCT), defined as a negative at 100 g oral glucose tolerance test. We determined the association between uterine artery PI and neonate weight. Observational study between 2010 and 2011. We examined the pulsatile index of both uterine arteries after 20 weeks` gestation. We defined the control group less than 135 mg/dL and hyperglycemic group above 135 mg/dL at GCT, but defined as a negative at OGTT. We categorized the mean PIs of both uterine arteries into 3 grades according to criteria of 5 percentile and 95 percentile for gestational age. We compared pregnancy outcomes between two groups and analyzed the association between birth weight and grade of pulsatile index in two groups. Hyperglycemic group(n=42) compared with controls(n=53) showed significantly decreased gestational age at delivery (37.5±1.8 vs. 38.4±1.6, p=0.010) and significantly increased GCT result (150.1±17.0 vs. 108.3±13.8, p<0.001) and rate of preterm delivery (6/42 vs. 3/53, p=0.044). Uterine artery PIs of hyperglycemia group was non-significantly higher (0.81± 0.31 vs. 0.75±0.34, p=0.352). In the neonate outcomes, hyperglycemic group showed significantly increased risk at hypoglycemia (4/42 vs. 0/53, p=0.022) and assisted ventilation (7/42 vs. 1/53, p=0.010). Neonate birth weight was not different between groups. A significant negative correlation was found between uterine artery PIs and birth weight (spearman`s rho = -0.233, p=0.023), however there was no association between PIs and GCT result. Pregnancy with borderline hyperglycemia at GCT, defined as a negative at OGTT, has increased risk at preterm delivery and adverse neonatal morbidities. Uterine arteries PIs, independently of the GCT result, significantly have the negative correlation with neonate birth weight.