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> 한국미생물생명공학회(구 한국산업미생물학회) > Journal of Microbiology and Biotechnology > 23권 1호

"A La Recherche" of Functions for the Spore Protein SASP-E from Bacillus subtilis

"A La Recherche" of Functions for the Spore Protein SASP-E from Bacillus subtilis

( Ruzal Sandra M ) , ( Patricia L. Bustos ) , ( Carmen Sanchez Rivas )

- 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)

- 발행년도 : 2013

- 간행물 : Journal of Microbiology and Biotechnology, 23권 1호

- 페이지 : pp.15-21 ( 총 7 페이지 )


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초록(외국어)
We previously observed that Bacillus subtilis spores from sspE mutants presented a lower germination capacity in media containing high salt concentrations (0.9M NaCl). This deficiency was attributed to the absence of SASP-E (gamma-type small-acid-soluble protein), rich in osmocompatible amino acids released by degradation. Herein we observed that, in addition, this mutant spore presented a reduced capacity to use L-alanine as germinant (L-ala pathway), required longer times to germinate in calcium dipicolinate (Ca2+-DPA), but germinated well in asparagine, glucose, fructose, and potassium chloride (AGFK pathway). Moreover, mild sonic treatment of mutant spores partially recovered their germination capacity in L-ala. Spore qualities were also altered, since sporulating colonies from the sspE mutant showed a pale brownish color, a higher adherence to agar plates, and lower autofluorescence, properties related to their spore coat content. Furthermore, biochemical analysis showed a reduced partition in hexadecane and a higher content of Ca2+-DPA when compared with its isogenic wild-type control. Coat protein preparations showed a different electrophoretic pattern, in particular when detected with antibodies against CotG and CotE. The complementation with a wild-type sspE gene in a plasmid allowed for recovering the wild-type coat phenotype. This is the first report of a direct involvement of SASP-E in the spore coat assembly during the differentiation program of sporulation.

논문정보
  • - 주제 : 자연과학분야 > 생물
  • - 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
  • - 간행물 : Journal of Microbiology and Biotechnology, 23권 1호
  • - 발행년도 : 2013
  • - 페이지 : pp.15-21 ( 총 7 페이지 )
  • - UCI(KEPA) : I410-ECN-0102-2013-470-002360166
저널정보
  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1017-7825
  • - 수록범위 : 1991–2021
  • - 수록 논문수 : 5624