In this study, we investigated antioxidative activity, antibacterial activity, nitric oxide (NO) production inhibitory activity and tyrosinase inhibitory activity of the methanol extract and its fractions from Puerariae Flos. The polyphenol and flavonoid content of the ethylacetate fraction of the extract was 142.47 mg/g and 42.86 mg/g as the highest values respectively. In the measurement of 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging ability and NO production inhibitory activity, the ethylacetate fraction of the extract was exhibited higher effect than other samples. Moreover, tyrosinase inhibitory activity of the ethylacetate fraction and butanol fraction of the extract showed higher activity than arbutin used as positive control. On the other hand, in antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa by disc diffusion assay, the ethylacetate fraction of the extract showed the highest activity. In conclusion, the ethylacetate fraction of the methanol extract from Puerariae Flos could be applicable to functional materials for skin-related fields such as whitening, anti-inflammation against external stimuli and infection.