Chitosan is an attractive biomaterial for wound care because of its biocompatibility and intrinsic hemostatic and antimicrobial properties. Honey has been used since ancient times for the treatment of some respiratory diseases and for the healing of skin wounds. In this study, we investigated the effect of mixture of oligochitosan and herbal honey(chito/H) on atopic dermatitis treatment. The effect of chito/H was analyzed by clinical skin features of atopic dermatitis skin lesions, measuring the serum lgE, and investigated skin surface using an optical microscope. The scores of skin symptom level and the serum IgE were decreased after treatment with chito/H compared with durg as a control group. These results suggest that the chito/H may be an alternative substance for the treatment of atopic dermatitis.