Recently single port access laparoscopic surgery has been reported since it has lower incisional morbidity and better cosmetic outcome compared to conventional laparoscopic surgery. We performed transumbilical single port access laparoscopic ovarian cystectomy, myomectomy and appendectomy at the same time in one patient using a specially designed single three-channel port that left virtually no scar. We used an Alexis(R) wound retractor (Applied Medical, CA, USA) and a surgical glove as the single three-channel port. The single port access laparoscopic gynecologic operation can be used feasibly in variable gynecologic condition without complication.