Prior to systemic investigation on intense husbandry environment-mediated stress outcome in pig or hen, we compared serum levels of epinephrine, norepinephrine, and cortisol in pigs or hens housed in intense husbandry confinement building or environmentally friendly husbandry facility. Pigs were accommodated under density of 3 per 3.3 ㎡and were kept on wet floors in the intense husbandry confinement building with fan-ventilation system. Meanwhile, the environmentally friendly husbandry facility with open ventilation system accommodated 2 pigs per 3.3 ㎡and pigs were kept on dry floors. Hens were housed under density of 3 per 0.1 ㎡in wire-partitioned battery cage at the intense husbandry confinement building with fan-ventilation system. Meanwhile, the environmentally friendly husbandry facility with open ventilation system accommodated 11 hens per 3.3 ㎡on flat ground. Five animals from each facility were randomly chosen and their peripheral blood was collected from jugular vein in pig or wing vein in hen. Levels of epinephrine, norepinephrine, and cortisol were determined using commercially available ELISA kit. Pigs housed in the intense husbandry confinement building demonstrated 10-fold and 6-fold lower level of epinephrine and norepinephrine, respectively, than those in the environmentally friendly husbandry facility. Furthermore, level of cortisol was significantly lowered in both pigs and hens housed in the intense husbandry confinement building, compared with that in the environmentally friendly husbandry facility. Overall our results suggest that pigs and hens housed in the intense husbandry confinement building are not adequately responding to extrinsic stress such as manual bleeding, eventually leading to vulnerability to various diseases.