The purpose of this study was to investigate the effect of long-term L-Carnosine Intake and regular aerobic exercise on blood MDA concentration and SOD, GPx, CK, LDH activity, regarding oxidative stress during exercise test for 30min (running at 16-17min). The rats were assigned randomly to 4 groups (control, n = 7; camosine intake, n = 7; exercise, n = 7; exercise + camosine intake, n = 7). Each regimen was 8 weeks long. MDA concentrations in exercise + camosine intake group was significantly lower compared to control group. SOD activity in exercise + carnosine intake group was significantly higher compared to the groups. GPx activity in exercise + camosine intake group was significantly higher compared to control group. CK activity in exercise and camosine intake, exercise + carnosine intake groups was lower compared to control group. LDH activity in exercise + carnosine intake group was significantly lower compared to the groups. These results indicated that long-time regular aerobic exercise and camosine intake may inhibit lipid peroxidation to oxidative stress and increase activity of SOD and GPx, but not CK and LDH