Silymarin and curcumin have been used for supportive treatment of liver disease of different etiology due to their hepatoprotective activities. The present study was carried out to investigate the hepatoprotective effects of silymarin and/or curcuma extract against hepatotoxins induced liver injury. To investigate hepatoprotective effects, the silymarin and/or curcuma extract were pre-treated orally to experimental animals. And thereafter a single dose of hepatotoxin, carbon tetrachloride (CCl_4) and acetaminophen were administered through oral or intraperitoneal route, respectively. Chronic liver damage was induced by subcutaneous injection of CCl_4 for 3 weeks (2 times/week). Hepatoprotective and therapeutic effects were monitored by estimating serum ALT and AST levels and by measuring hepatic glutathione (GSH) and malondialdehyde (MDA) levels. Collagen type 1 was detected with immunostaining to assess fibrosis. The results showed that the mixture of silymarin and curcuma extract significantly reduced serum biochemistry levels and MDA levels compared with those of control group in both acute and chronic animal models. In antifibrotic effect, the relative hepatic collagen content was significantly decreased by silymarin and/or curcuma extract treatment. It was concluded that the complex of silymarin and curcuma extract have a both hepatoprotective and therapeutic effect synergically in rat liver injury induced by heptotoxins.