A intronless pseudogene for human phenylethanolamine N-methyl transferase (PNMT), terminal enzyme catalysing the final step in the catecholamine biosynthetic pathway, was isolated from a human recombinant DNA library by hybridization with a bovine cDNA probe and characterized in detail. This gene has five distinct sequence characteristics found in processed pseudogene. First, this gene lacks completely the intervening sequences. Second, this gene is truncated at the 5’ end peptide encoding region by 433 base pairs. Third, the 502 base paris of this gene containing poly(A) singnal are completely identical to the 3` half of mRNA encoding region of functional gene. Fourth, this has a poly(A) tail. Fifth, this gene is flanked by direct repeat of 6 base pairs. Here we report the complete sequence of a human pseudogene for phenylethanolamine N-methyltransferase and this is the first report of cloning of pseudogene for catecholamine biosynthetic enzymes.