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> 한국미생물생명공학회(구 한국산업미생물학회) > Journal of Microbiology and Biotechnology > 25권 12호

Ice-Binding Protein Derived from Glaciozyma Can Improve the Viability of Cryopreserved Mammalian Cells

Ice-Binding Protein Derived from Glaciozyma Can Improve the Viability of Cryopreserved Mammalian Cells

( Hak Jun Kim ) , ( Hye Eun Shim ) , ( Jun Hyuck Lee ) , ( Yong Cheol Kang ) , ( Young Baek Hur )

- 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)

- 발행년도 : 2015

- 간행물 : Journal of Microbiology and Biotechnology, 25권 12호

- 페이지 : pp.1989-1996 ( 총 8 페이지 )


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초록(외국어)
Ice-binding proteins (IBPs) can inhibit ice recrystallization (IR), a major cause of cell death during cryopreservation. IBPs are hypothesized to improve cell viability after cryopreservation by alleviating the cryoinjury caused by IR. In our previous studies, we showed that supplementation of the freezing medium with the recombinant IBP of the Arctic yeast Glaciozyma sp. (designated as LeIBP) could reduce post-thaw hemolysis of human red blood cells and increase the survival of cryopreserved diatoms. Here, we showed that LeIBP could improve the viability of cryopreserved mammalian cells. Human cervical cancer cells (HeLa), mouse fibroblasts (NIH/3T3), human preosteoblasts (MC3T3-E1), Chinese hamster ovary cells (CHO-K1), and human keratinocytes (HaCaT) were evaluated. These mammalian cells were frozen in dimethyl sulfoxide (DMSO)/fetal bovine serum (FBS) solution with or without 0.1 mg/ml LeIBP at a cooling rate of -1℃/min in a -80℃ freezer overnight. The minimum effective concentration (0.1 mg/ml) of LeIBP was determined, based on the viability of HeLa cells after treatment with LeIBP during cryopreservation and the IR inhibition assay results. The post-thaw viability of mammalian cells was examined. In all cases, cell viability was significantly enhanced by more than 10% by LeIBP supplementation in 5% DMSO/5% FBS: viability increased by 20% for HeLa cells, 28% for NIH/3T3 cells, 21% for MC3T3-E1, 10% for CHO-K1, and 20% for HaCaT. Furthermore, addition of LeIBP reduced the concentrations of toxic DMSO and FBS down to 5%. Therefore, we demonstrated that LeIBP can increase the viability of cryopreserved mammalian cells by inhibiting IR.

논문정보
  • - 주제 : 자연과학분야 > 생물
  • - 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
  • - 간행물 : Journal of Microbiology and Biotechnology, 25권 12호
  • - 발행년도 : 2015
  • - 페이지 : pp.1989-1996 ( 총 8 페이지 )
  • - ECN : ECN-0102-2016-470-000196371
  • - UCI : I410-ECN-0102-2016-470-000196371
저널정보
  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1017-7825
  • - 수록범위 : 1991–2018
  • - 수록 논문수 : 5087