This study measured the anti-inflammatory and basic physiological activities of its immune mechanism of Palmarosa essential oil, Myrrh essential oil, and Sandalwood essential oil which can be used for functional materials in the beauty care industry. The experimental results showed that Palmarosa essential oil, Myrrh essential oil, and Sandalwood essential oil had no toxicity in RAW264.7 cell. Then, NO inhibition of Palmarosa essential oil, Myrrh essential oil, and Sandalwood essential oil was assessed by LPS-inducement. The result showed that the LPS-treated group had a remarkable increase in NO concentration and the Palmarosa essential oil, Myrrh essential oil, and Sandalwood essential oil-treated group(500 ug/mL) had excellent NO inhibition. In particular, the Sandalwood essential oil-treated group had significant NO inhibition. Based on the results, Palmarosa essential oil, Myrrh essential oil, and Sandalwood essential oil had excellent NO inhibition induced by LPS in RAW264.7 cell. In particular, the most excellent NO inhibition was found in Sandalwood essential oil, followed by Myrrh essential oil and Palmarosa essential oil.