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> 한국미생물생명공학회(구 한국산업미생물학회) > Journal of Microbiology and Biotechnology > 23권 8호

Molecular Cloning and Enzymatic Characterization of Cyclomaltodextrinase from Hyperthermophilic Archaeon Thermococcus sp. CL1

Molecular Cloning and Enzymatic Characterization of Cyclomaltodextrinase from Hyperthermophilic Archaeon Thermococcus sp. CL1

( Jae Eun Lee ) , ( In Hwan Kim ) , ( Jong Hyun Jung ) , ( Dong Ho Seo ) , ( Sung Gyun Kang ) , ( James F Holden ) , ( Jae Ho Cha ) , ( Cheon Seok Park )

- 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)

- 발행년도 : 2013

- 간행물 : Journal of Microbiology and Biotechnology, 23권 8호

- 페이지 : pp.1060-1069 ( 총 10 페이지 )


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논문제목
초록(외국어)
Genome organization near cyclomaltodextrinases (CDases) was analyzed and compared for four different hyperthermophilic archaea: Thermococcus, Pyrococcus, Staphylothermus, and Thermofilum. A gene (CL1_0884) encoding a putative CDase from Thermococcus sp. CL1 (tccd) was cloned and expressed in Escherichia coli. TcCD was confirmed to be highly thermostable, with optimal activity at 85oC. The melting temperature of TcCD was determined to be 93oC by both differential scanning calorimetry and differential scanning fluorimetry. A size-exclusion chromatography experiment showed that TcCD exists as a monomer. TcCD preferentially hydrolyzed α-cyclodextrin (α-CD), and at the initial stage catalyzed a ring-opening reaction by cleaving one α-1,4-glycosidic linkage of the CD ring to produce the corresponding single maltooligosaccharide. Furthermore, TcCD could hydrolyze branched CDs (G1-α-CD, G1-β- CD, and G2-β-CD) to yield significant amounts (45%, 40%, and 46%) of isomaltooligosaccharides (panose and 62-α-maltosylmaltose) in addition to glucose and maltose. This enzyme is one of the most thermostable maltogenic amylases reported, and might be of potential value in the production of isomaltooligosaccharides in the food industry.

논문정보
  • - 주제 : 자연과학분야 > 생물
  • - 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
  • - 간행물 : Journal of Microbiology and Biotechnology, 23권 8호
  • - 발행년도 : 2013
  • - 페이지 : pp.1060-1069 ( 총 10 페이지 )
  • - UCI(KEPA) : I410-ECN-0102-2014-400-001976833
저널정보
  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1017-7825
  • - 수록범위 : 1991–2019
  • - 수록 논문수 : 5308