Background: Gallbladder (GB) polyps are frequently encountered in nowadays. Previous studies have reported male sex, chronic hepatitis B infection, low HDL-cholesterol level and obesity as the risk factors associated with GB polyp. The aim of this study was to identify the risk factors of GB polyp in healthy subjects. Methods: Patients who underwent examination through health promotion in Yeungnam university hospital from Jan 2010 to Dec 2013 were included. All patients underwent abdominal ultrasonography and diagnosis of GB polyp was made by abdominal ultrasonography. Their medical records were reviewed and analyzed retrospectively. Results: A total of 23,899 subjects were included in this study and 1,973 subjects were diagnosed with GB polyp. Mean age of GB polyp group was signifi cantly higher than non-GB polyp group (51.1 ± 10.4 vs 49.9 ± 11.4, p-value=0.001). Male was more predominant in GB polyp group (71.1% vs 54.8%, p<0.001). On univariate analysis, older age, male sex, HDL-cholesterol level, presence of fatty liver, GB stone and higher body mass index were signifi cantly associated with presence of GB polyp. On multivariate analysis, male sex (odds ratio=1.909, p<0.001), low HDL-cholesterol level (odds ratio=0.996, p=0.019) and GB stone (odds ratio=2.046, p<0.001) were identifi ed as the risk factors associated with GB polyp. Conclusions: In our study, risk factors for GB polyp were male sex, low HDL-cholesterol level and GB stone.