Aims: M.Colombo, W.Lange studies showed that 30-40% of people become chronic after suffering from-Hepatitis B and C virus, about 50% chronic cases transformed into primary liver cancer. There are a few studies in our country were conducted on hepatitis among health care professionals, particular nursing personnel. The study was conducted to identify of hepatitis B and C virus among nurses and make recommendations to prevent and control of hepatitis B and C virus infections. Methods: We carried out cross-sectional study among selected nurses to determine surface antigen of hepatitis B virus and antibodies to hepatitis C virus. For identification of these antibody and antigen and validation of results ELISA tests from CTK, Biotech company (USA) and simplifying diagnostics were used. Results: There were 598 nurses from the First Central hospital, the Second Central hospital, the Third central hospital, Railway Central hospital, Hospital Ministry of Justice and Internal Affairs and National Center of Maternal and Child Health who participated in the study. From 5 hospitals 598 nurses surveyed and revealed the hepatitis B virus surface antigen positive 18.9%, hepatitis C virus antibodies in 23.1%, co-infection of hepatitis B and C were detected 1.2%. There is an urgent need to provide knowledge to medical personnel regarding standards during the procedures, concerning hepatitis infections monitoring and improve technology used during procedures. Conclusions: The study identified that 43.2% of nurses surveyed on hepatitis B and C viruses were detected. It shows a high prevalence among the nurses in Mongolia.